Rabbit TNF-Beta ELISA Kit
SKU: 98790537008

Rabbit TNF-Beta ELISA Kit

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Description

Rabbit TNF-Beta ELISA KitThe Rabbit TNF Beta ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rabbit TNFb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rabbit TNFb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only

The Rabbit TNF-Beta ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit TNFb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit TNFb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit TNFb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit TNFb in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 98790537008

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