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Testosterone ELISA KitThe Testosterone EIA kit is based on the principle of competitive binding between Testosterone in the test specimen and Testosterone HRP conjugate for a constant amount of mouse anti Testosterone. In the incubation, mouse anti Testosterone coated wells are incubated with 25ul of Testosterone standards, controls, patient samples, 100 ul Testosterone HRP conjugate reagent at room temperature for 60 minutes. During the incubation, a fixed amount of HRP
The Testosterone EIA kit is based on the principle of competitive binding between Testosterone in the test specimen and Testosterone-HRP conjugate for a constant amount of mouse anti-Testosterone. In the incubation, mouse anti-Testosterone coated wells are incubated with 25ul of Testosterone standards, controls, patient samples, 100 ul Testosterone-HRP conjugate reagent at room temperature for 60 minutes. During the incubation, a fixed amount of HRP-labeled Testosterone competes with the endogenous Testosterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific Testosterone antibody. Thus, the amount of Testosterone peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of Testosterone in the specimen increases. Unbound Testosterone peroxidase conjugate is then removed and the wells washed. Next, a solution of TMB Reagent is then added and incubated at room temperature for 15 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450nm.
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4.1 ★★★★★
Based on 1278 reviews
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Product Reviews
★★★★★ 5
Superman, Superboy, Supergirl, Krypto, a little of everything.
"Superman in the Fifties" is a collection of 17 stories from 1950 to 1959 reprinted in color. For me it was a nostalgic return to when I was a child and able to purchase 10 comics for a dollar off a turning rack (no sales tax either) at the local soda shop. The comics from the fifties served as an escapism for much of the youth at the time that was coming of age with the awareness that we could be threatened by nuclear attack at any time. Bomb shelter construction could be found in Popular Science magazines, and drills were performed in schools with students going into halls and crouching down and covering their heads or hiding under desks.
A hero was needed, one with super powers who could make the world right. We could all dream. Superman, "Faster than a speeding bullet, more powerful than a locomotive, able to leap tall buildings in a single bound...fighting a never ending battle for truth, justice, and the American way." Sorry, just had to put that TV intro in from the 50's.
The stories may seem dated, but that is part of the mystique of reading comics that are reprinted.
The titles in this volume are:
- Three Supermen From Krypton!
- The Menace From The Stars!
- The Girl Who Didn't Believe In Superman!
- Superboy's Last Day In Smallville!
- The Ugly Superman!
- Superman's Big Brother!
- The Super-Dog From Krypton!
- Titano, The Super-Ape!
- The Supergirl From Krypton!
- Superman's Super-Magic Show!
- The Super-Duel In Space
- The Battle With Bizarro!
- The Bride Of Bizarro!
- The End Of The Planet!
- Superman And Robin!!
- The Stolen Superman Signal
- The Girl In Superman's Past
My favorite was "The Super Duel in Space" with Brainiac reducing some of Earth's cities and putting them in bottles. It was a good change up from the usual fiction, and non-fiction that I read. It provides plenty of background about Superman for young readers with terrific art that was hand drawn, unlike most modern comics that are created on computers. The stories are a very good sampling of "Superman" from the fifties.
I look forward to reading "Superman in the Sixties."
I give "Superman in the Fifties" 5 stars.
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Reviewed in the United States on September 15, 2011
★★★★★ 5
This is perfect - I just wish they would reprint all of the 1950's and 60's stories!
This is great - wish I had more Superman stories from the 1950's to read!!
I was born in 1952 so this is something right up my alley. I am a big Superman fan and had most of the mid to late 1950'2 issues until my mom burned them when I went away to college. I understand why but still wish I had them - for many different reasons.
The coloring is bright and spot-on. I even remember most of these stories - I know it's been more than 50 years ago but they were a big part of my early life. All of the stories are least good and couple are truly great. Some of the plots are little silly by todays standards but things have most definitely changed since they were written.
IF you love Superman, grew-up in the 50's or just anything historical you will love this! I just wish they would reprint all the 50's and 60's stories. That is something I would most certainly buy!!
My rating for this is a perfect 10 out of 10 - for me it's perfect - all I want is MORE of this!!
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Reviewed in the United States on June 21, 2016
★★★★★ 5
Superman in the wacky '50s
Format: Paperback
Love the Jerry Ordway cover, although an image from the era would have been more fitting. This may be the Superman era most of remember best, the gimmick-laden Weisinger era that made the most contributions to Superman lore. For me the best story here is the first one, in which the other survivors of Krypton's demise -- Kryptonian (here called "Kryptonites") super criminals U-Ban, Mala and Kizo -- appear for the first time. Other firsts include the first Brainiac tale, Supergirl's first appearance, and "The Last Superman of Krypton." Krypton did not play a big role in 1940s Superman stories. Curiously, Brainiac looks as we've come to know him on the cover of that comic, but less so inside. This is a decent sampling of '50s Superman tales. Much here to enjoy!
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Reviewed in the United States on May 23, 2025
★★★★★ 4
A great read
Format: Kindle
Great stories from the fifties that I did not know existed until I opened this book. I hope there are more stories to come.
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Reviewed in the United States on December 8, 2023
★★★★★ 5
Just for Fun
You can't help, in reading these stories, remarking on how comics (and popular culture in general) reflect what we want on our minds and how we want to feel about ourselves.
Superman today is intense. He fights apocalyptic battles, and he sometimes loses! There's a lot at stake -- everything, EVERYTHING, lies in the balance. Superman himself seems literaly beyond human. In order to live the life of challenges he faces, he must be beyond the concerns of everyday life -- he can't really share in the life that the rest of us live.
Superman in the fifties lived in a much more comfortable, stable world, and his own life was much more continuous with ours. In these stories, he discovers that he is not alone -- his long last pal, Krypto, shows up, and he discovers his cousin, Supergirl. He has girlfriends -- Lana Lang and Lois Lane compete for his attention (without a lot of the psychological anxiety that Superman will face in the future over his inability to live a normal life and raise a normal family).
The villains, like Lex Luthor, aren't even purely evil -- they have their limits. Bizarro is not evil at all, just . . . dumb and amusing so long as Superman can repair any damage he does.
It's a little bit trivial to point out how comics reflect cultural reality, but . . . they do. It's fun to revisit the fifties here -- i suspect it's not so much an innocent age as one in which the story we told ourselves about ourselves (as in our Superman comics) was focused where we wanted it to be focused -- family, friends, the pleasures of everyday life.
But, putting aside all the sociology and pretenses of cultural history, these stories are just fun to read. It's not the Superman we know now, it's just different, a change of pace, fun.
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Reviewed in the United States on September 28, 2013
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