Human CTX-III (Cross Linked C-telopeptide of Type III Collagen) ELISA Kit

Human CTX-III (Cross Linked C-telopeptide of Type III Collagen) ELISA KitThe Human CTX III (Cross Linked C telopeptide of Type III Collagen) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Human CTX III. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Human CTX III. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.

The Human CTX-III (Cross Linked C-telopeptide of Type III Collagen) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human CTX-III. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Human CTX-III. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CTX-III in the samples is then determined by comparing the OD of the samples to the standard curve.