Bovine TNF-Alpha (Tumor Necrosis Factor Alpha) ELISA Kit
SKU: 36136399957

Bovine TNF-Alpha (Tumor Necrosis Factor Alpha) ELISA Kit

$202.50 $225.00
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Ships within 48 hours · Estimated delivery Jul 4 - Jul 9

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Description

Bovine TNF-Alpha (Tumor Necrosis Factor Alpha) ELISA KitThe Bovine TNF Alpha (Tumor Necrosis Factor Alpha) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Bovine TNF Alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Bovine TNF Alpha and Avidin Horseradish Peroxidase (HRP) conjugate are added

The Bovine TNF-Alpha (Tumor Necrosis Factor Alpha) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Bovine TNF-Alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Bovine TNF-Alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Bovine TNF-Alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Bovine TNF-Alpha. You can calculate the concentration of Bovine TNF-Alpha in the samples by comparing the OD of the samples to the standard curve.

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SKU: 36136399957

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4.2 ★★★★★
Based on 1677 reviews
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Desert Rat X
Lowell, US
★★★★★ 5
Great for a medium chewer!
Flavor Name: Bacon, Size: Medium
Bought this for my Standard Poodle. He's a year old, 65 pounds, and loves to chew but doesn't have the jaw strength of some of my previous dogs (I think my pit bull would have destroyed this in short order!). This works well for him; it's his favorite toy for fetching. It bounces in an unpredictable manner when you throw it so he has fun chasing and retrieving it. It is heavy so be careful throwing it in the house! I like these shapes better than balls that will roll far under things and be difficult to retrieve. I don't know that I would leave him alone with it in case he did chew a piece off, but for monitored play I highly recommend. I'll definitely buy more if these ever wear out but I don't see that happening in the near future!
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Reviewed in the United States on January 14, 2018
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debbie
Cuba, US
★★★★★ 2
Smells like rubber.
Flavor Name: Bacon, Size: Medium
I bought this flavored nylabone for my dog because he had one that he just loved and it finally had to be thrown out. So I bought him this one and he won't even chew on it. All it smells like is rubber. No flavor smell AT ALL. But our other dog chews anything and he chews this so I kept it. I won't buy nylabone again.
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Reviewed in the United States on September 27, 2024
E
Verified Purchase
El James
San Leandro, US
★★★★★ 5
Super chewer approved
Flavor Name: Peanut Butter, Size: Large
One of the few safe chewy bones for a super chewer. Very durable, an appropriate price, and a good size for our 70lb pitty.
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Reviewed in the United States on December 11, 2025
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jvonbernard
Natrona Heights, US
★★★★★ 5
The best toys for dogs I could ever find
Size: X Large
I have bull terriers. They chew wood for fun... But this brand in the appropiate size and in any shape has been the best thing for them! They last for a very long time (they have not even dented them and they've had one (another shape) for the last two years. Now I bought these bones so that they can play together, each graving one side of it. They have so much fun! They are worth every penny.
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Reviewed in the United States on March 19, 2026
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Verified Purchase
tammi pasquale
Boise, US
★★★★★ 5
Best chew toy
Size: Medium (Pack of 1)
Kong toys are the best. They hold up through the heavy chewing stage. Definitely recommend if you have a heavy chewer.
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Reviewed in the United States on May 28, 2026

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