Rat T (Testosterone) ELISA Kit

Rat T (Testosterone) ELISA KitThis Rat T (Testosterone) ELISA Kit uses the competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Rat T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat T are added to the micro ELISA plate wells. Rat T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate

This Rat T (Testosterone) ELISA Kit uses the competitive ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat T are added to the micro ELISA plate wells. Rat T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450+/-2 nm. The concentration of Rat T in the samples is then determined by comparing the OD of the samples to the standard curve.