Canine Procalcitonin ELISA

Canine Procalcitonin ELISAThe Canine Procalcitonin ELISA is an enzyme immunoassay for the quantitative determination of Procalcitonin in canine serum, plasma and urine. In the Canine Procalcitonin ELISA kit, standards and samples are incubated in microtitration wells pre coated with polyclonal anti canine procalcitonin antibody. After 60 minutes incubation followed by washing, biotin labelled polyclonal anti canine procalcitonin antibody is added and incubated with the

The Canine Procalcitonin ELISA is an enzyme immunoassay for the quantitative determination of Procalcitonin in canine serum, plasma and urine.

In the Canine Procalcitonin ELISA kit, standards and samples are incubated in microtitration wells pre-coated with polyclonal anti-canine procalcitonin antibody. After 60 minutes incubation followed by washing, biotin-labelled polyclonal anti-canine procalcitonin antibody is added and incubated with the captured procalcitonin for 60 minutes. After another washing, streptavidin-HRP conjugate is added. After 30 minutes incubation and the last washing step, the remaining conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured. The absorbance is proportional to the concentration of canine procalcitonin. A standard curve is constructed by plotting absorbance values against procalcitonin concentrations of standards and concentrations of unknown samples are determined using this standard curve.