FGF-23 ELISA

FGF-23 ELISAThe Intact Fibroblast Growth Factor 23 (FGF 23) ELISA is an enzyme immunoassay for the quantitative determination of Intact Fibroblast Growth Factor 23 (FGF 23) in human EDTA plasma. The microtiter plate is coated with the antibody specifically binding Fibroblast growth factor 23. The human EDTA plasma is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with biotin labelled

The Intact Fibroblast Growth Factor 23 (FGF-23) ELISA is an enzyme immunoassay for the quantitative determination of Intact Fibroblast Growth Factor 23 (FGF-23) in human EDTA plasma.

The microtiter plate is coated with the antibody specifically binding Fibroblast growth factor 23. The human EDTA plasma is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with biotin-labelled detection antibody. Following another washing step, Streptavidin-HRP conjugate is added into the well. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of FGF23 in the specimen. The concentration of FGF23 in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.